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| Product Name | Murine IP-10 ELISA Kit |
| Catalog No. | EC-0019 |
| Description | Murine IP-10 ELISA kit is a kit for the in vitro detection of murine IP-10 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Specificity | Recognizes both natural and recombinant murine IP-10. |
| Application | It can be used for immediate in vitro detection of murine IP-10. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Chemokines play a critical role in regulating immune cell migration and activation, and Interferon-Gamma-Inducible Protein 10 (IP-10, also known as CXCL10) stands out as a key member of this family—especially in murine (mouse) model research, which is widely used in immunology, infectious diseases, and inflammatory disorder studies.
Murine IP-10 is primarily induced by interferon-gamma (IFN-γ), a major pro-inflammatory cytokine, and is secreted by a variety of cells including macrophages, dendritic cells, endothelial cells, and fibroblasts. Its core biological functions include:
Recruiting activated T cells, natural killer (NK) cells, and monocytes to sites of inflammation, which is essential for orchestrating adaptive and innate immune responses.
Mediating anti-viral immunity by enhancing the accumulation of immune cells in infected tissues, making it a key marker for studying viral infections (e.g., murine models of influenza, COVID-19, or herpesvirus).
Regulating inflammatory processes in chronic conditions such as autoimmune diseases (e.g., murine models of rheumatoid arthritis, multiple sclerosis) and tissue damage (e.g., ischemia-reperfusion injury), where abnormal IP-10 expression is closely linked to disease severity.
In preclinical research, accurate detection of murine IP-10 levels is critical for:
Validating the efficacy of immunotherapies (e.g., checkpoint inhibitors, cytokine-based treatments) by monitoring IP-10 as a surrogate marker of immune activation.
Characterizing disease progression in murine models of inflammation—for example, tracking IP-10 changes in serum or tissue homogenates to assess the impact of experimental drugs on inflammatory responses.
Investigating pathogen-host interactions, such as measuring IP-10 secretion in murine macrophages infected with bacteria (e.g., Mycobacterium tuberculosis) or parasites (e.g., Plasmodium).
Traditional methods for detecting murine IP-10 (e.g., Western blotting, flow cytometry) often suffer from limitations such as low throughput, high sample consumption, or complex operation. Enzyme-Linked Immunosorbent Assay (ELISA) has emerged as the gold standard for murine IP-10 detection due to its high specificity, sensitivity, and ease of use—making it ideal for high-throughput screening of multiple samples (e.g., 96-well plate formats) in research laboratories.
Our Murine IP-10 ELISA Kit (Cat.No: EC-0019) is specifically designed to address the needs of preclinical researchers, providing a reliable in vitro tool to quantify murine IP-10 in biological samples. It builds on the proven performance of ELISA technology while optimizing for specificity, sample efficiency, and workflow convenience—supporting a wide range of research applications in immunology and disease modeling.
Broad Specificity for Murine IP-10 Forms: Recognizes both natural murine IP-10 (endogenously secreted by murine cells) and recombinant murine IP-10 (often used as a standard in experimental setups), ensuring compatibility with diverse research workflows (e.g., comparing endogenous IP-10 in disease models vs. recombinant IP-10 in drug stimulation assays).
Versatile Sample Compatibility: Optimized for detecting murine IP-10 in common biological samples used in preclinical research, including serum, plasma, cell culture supernatants (e.g., from murine macrophages or T cell cultures), and tissue homogenates (e.g., from murine lung, spleen, or joint tissues).
Convenient Storage and Transportation: Requires only refrigerated storage at 4°C (no need for -20°C or -80°C freezers), reducing the risk of reagent degradation during long-term storage; supports room-temperature transportation, minimizing logistics challenges and costs for international or remote research teams.
Ready-to-Use Format: Comes with pre-optimized reagents (e.g., capture antibodies, detection antibodies, enzyme conjugates, substrates) and a detailed protocol, eliminating the need for time-consuming reagent preparation and reducing the learning curve for new users.
High Throughput Compatibility: Available in 1×96 T and 2×96 T formats, allowing researchers to process 96 or 192 samples per kit—ideal for large-scale experiments (e.g., screening multiple time points, dose groups, or experimental conditions in a single assay run).
Superior Detection Accuracy: Minimizes cross-reactivity with other murine chemokines (e.g., CXCL9, CXCL11, or IL-8) and non-target proteins in biological samples, ensuring that detected signals specifically reflect murine IP-10 levels—critical for avoiding false-positive or false-negative results in data interpretation.
Low Sample Consumption: Requires only a small volume of sample (typically 10–50 μL per well, depending on sample type) to perform a detection, preserving precious samples (e.g., limited serum from murine models with low blood volume or rare tissue homogenates) for additional downstream experiments.
Rapid Assay Turnaround: Enables complete detection (from sample preparation to result reading) in approximately 3–4 hours, significantly faster than traditional methods like Western blotting (which can take 6–8 hours) or Luminex multi-factor assays (which may require longer incubation times for multi-analyte detection).
Reliable Batch-to-Batch Consistency: Manufactured under strict quality control standards, with batch-to-batch coefficient of variation (CV) < 10% for both standard curve precision and sample repeatability—ensuring that results from different kit batches are comparable, a key requirement for long-term research projects.
For research use only, not for clinical use.
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